European Molecular Biology Laboratory

Biologists from Europe amounted to a massive database, in which each human gene, which codes proteins that corresponds to videotape the process of cell division in suppressing the activity of this gene.

The researchers worked with cells HeLa - a popular line, derived from cervical cancer cells taken from Henrietta Lacks. This line is called "immortal", because such cells are able to share an infinite number of times.

In humans, as is known, about 21 000 genes encoding proteins. Authors suppressed the expression of individual genes from the known method of RNA interference and within two days, watched how the changes affect the process of cell division with a pre-labeled with fluorescent markers of chromosomes. As a result, they have registered more than 19 million divisions and created about 190,000 videos.

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Edit as much information manually is impossible, and scientists have been entrusted as a special sort of computer program that tracks the position of the cell nuclei and by assessing the shape, placed each file in one of 16 possible groups. As a result, biologists have identified a little less than 600 genes that can affect the process of mitosis (nuclear division with preservation of the number of chromosomes). Most of these genes have never been associated with mitosis.

Some genes were involved in the early stages of division and disrupt the functioning of others are in the later stages (at the stage of cytokinesis - division of cytoplasm). Because video files are grouped together, the authors could predict the mitotic functions of the "new" genes involved, for example, in the formation of spindle.

The data are summarized in an accessible throughout the base. "Scientists can come to the site, enter the name of the gene of interest to them and then see what happens in the suppression of its activity, - says study leader Jan Ellenberg of the European Molecular Biology Laboratory. - In addition, they provide information about genes that give a similar effect . And to learn it, do not have to spend months and years in the laboratory.